Trichoderma harzianum is a naturally occurring fungus, which is used as a biofungicide to protect mulberry (Morus alba L.) from root-rot and wilt diseases caused by other harmful fungi. A talc-based biofungiside (Raksha) has been developed using T. harzianum for the control of root rot diseases of mulberry. Raksha is applied after mixing with farm-yard manure (FYM) (1:50) @ 500 g mixture/plant in the root zone followed by irrigation. There are no expected health risks in human and residual toxicity in mulberry as well as silkworm from the use of product containing T. harzianum as active ingredient. The mass production and quality control methods for production of the biofungicide have been standardized.